Protocol to measure calcium spikes in cardiomyocytes obtained from human pluripotent stem cells using a ready-to-use media

The derivation of cardiomyocytes from human pluripotent stem cells (hPSCs) is a powerful tool to investigate early cardiogenesis and model diseases in vitro. Here, we present an optimized protocol to obtain contracting hPSCs-derived cardiomyocytes using a ready-to-use kit. We describe steps for hPSC...

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Detalles Bibliográficos
Autores principales: Astro, Veronica, Ramirez-Calderon, Gustavo, Adamo, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140149/
https://www.ncbi.nlm.nih.gov/pubmed/37060558
http://dx.doi.org/10.1016/j.xpro.2023.102252

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140149/
https://www.ncbi.nlm.nih.gov/pubmed/37060558
http://dx.doi.org/10.1016/j.xpro.2023.102252

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