Cargando…
Protocol to measure calcium spikes in cardiomyocytes obtained from human pluripotent stem cells using a ready-to-use media
The derivation of cardiomyocytes from human pluripotent stem cells (hPSCs) is a powerful tool to investigate early cardiogenesis and model diseases in vitro. Here, we present an optimized protocol to obtain contracting hPSCs-derived cardiomyocytes using a ready-to-use kit. We describe steps for hPSC...
Autores principales: | Astro, Veronica, Ramirez-Calderon, Gustavo, Adamo, Antonio |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140149/ https://www.ncbi.nlm.nih.gov/pubmed/37060558 http://dx.doi.org/10.1016/j.xpro.2023.102252 |
Ejemplares similares
-
Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes
por: Zhang, Joe Z., et al.
Publicado: (2021) -
Heart in a Dish: From Traditional 2D Differentiation Protocols to Cardiac Organoids
por: Ramirez-Calderon, Gustavo, et al.
Publicado: (2022) -
A simple protocol to produce mature human-induced pluripotent stem cell-derived cardiomyocytes
por: Knight, Walter E., et al.
Publicado: (2021) -
Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media
por: Kameda, Kotaro, et al.
Publicado: (2022) -
Protocol for germ lineage differentiation of primed human pluripotent stem cells using chemically defined, nutrient-balanced media
por: Lu, Vivian, et al.
Publicado: (2022)