Acceleration of CRISPR/Cas9-Mediated Editing at Multiple Sites in the Saccharomyces cerevisiae Genome

The application of the CRISPR/Cas9-based genome editing technique to the yeast Saccharomyces cerevisiae has made it possible to simultaneously modify several sites, particularly to integrate several expression cassettes. The existing methods provide high efficiency for such modifications; however, c...

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Detalles Bibliográficos
Autores principales: Karpukhin, Alexey D., Sabirzyanov, Fanis A., Serebrianyi, Vsevolod A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141985/
https://www.ncbi.nlm.nih.gov/pubmed/37104021
http://dx.doi.org/10.3390/mps6020039

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141985/
https://www.ncbi.nlm.nih.gov/pubmed/37104021
http://dx.doi.org/10.3390/mps6020039

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