Semi-automated optimized method to isolate CRISPR/Cas9 edited human pluripotent stem cell clones

BACKGROUND: CRISPR/Cas9 editing systems are currently used to generate mutations in a particular gene to mimic a genetic disorder in vitro. Such “disease in a dish” models based on human pluripotent stem cells (hPSCs) offer the opportunity to have access to virtually all cell types of the human body...

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Detalles Bibliográficos
Autores principales: Frank, Elie, Cailleret, Michel, Nelep, Constantin, Fragner, Pascal, Polentes, Jérome, Herardot, Elise, El Kassar, Lina, Giraud-Triboult, Karine, Monville, Christelle, Ben M’Barek, Karim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142500/
https://www.ncbi.nlm.nih.gov/pubmed/37106426
http://dx.doi.org/10.1186/s13287-023-03327-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142500/
https://www.ncbi.nlm.nih.gov/pubmed/37106426
http://dx.doi.org/10.1186/s13287-023-03327-2

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