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Semi-automated optimized method to isolate CRISPR/Cas9 edited human pluripotent stem cell clones

BACKGROUND: CRISPR/Cas9 editing systems are currently used to generate mutations in a particular gene to mimic a genetic disorder in vitro. Such “disease in a dish” models based on human pluripotent stem cells (hPSCs) offer the opportunity to have access to virtually all cell types of the human body...

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Detalles Bibliográficos
Autores principales:	Frank, Elie, Cailleret, Michel, Nelep, Constantin, Fragner, Pascal, Polentes, Jérome, Herardot, Elise, El Kassar, Lina, Giraud-Triboult, Karine, Monville, Christelle, Ben M’Barek, Karim
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2023
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142500/ https://www.ncbi.nlm.nih.gov/pubmed/37106426 http://dx.doi.org/10.1186/s13287-023-03327-2

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