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Online Monitoring of the Growth of Probiotic Bacteria and Metabolites in the Fermentation of a Teff Substrate Using Model-Based Calibration of 2D Fluorescence Spectra

The demand for probiotic bacteria-fermented food products is increasing; however, the monitoring of the fermentation process is still challenging when using conventional approaches. A classical approach requires a large amount of offline data to calibrate a chemometric model using fluorescence spect...

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Autores principales: Alemneh, Sendeku Takele, Babor, Majharulislam, Zettel, Viktoria, von Wrochem, Almut, Hitzmann, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143008/
https://www.ncbi.nlm.nih.gov/pubmed/37110455
http://dx.doi.org/10.3390/microorganisms11041032
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author Alemneh, Sendeku Takele
Babor, Majharulislam
Zettel, Viktoria
von Wrochem, Almut
Hitzmann, Bernd
author_facet Alemneh, Sendeku Takele
Babor, Majharulislam
Zettel, Viktoria
von Wrochem, Almut
Hitzmann, Bernd
author_sort Alemneh, Sendeku Takele
collection PubMed
description The demand for probiotic bacteria-fermented food products is increasing; however, the monitoring of the fermentation process is still challenging when using conventional approaches. A classical approach requires a large amount of offline data to calibrate a chemometric model using fluorescence spectra. Fluorescence spectra provide a wide range of online information during the process of cultivation, but they require a large amount of offline data (which involves laborious work) for the calibration procedure when using a classical approach. In this study, an alternative model-based calibration approach was used to predict biomass (the growth of Lactiplantibacillus plantarum A6 (LPA6) and Lacticaseibacillus rhamnosus GG (LCGG)), glucose, and lactic acid during the fermentation process of a teff-based substrate inoculated with mixed strains of LPA6 and LCGG. A classical approach was also applied and compared to the model-based calibration approach. In the model-based calibration approach, two-dimensional (2D) fluorescence spectra and offline substituted simulated data were used to generate a chemometric model. The optimum microbial specific growth rate and chemometric model parameters were obtained simultaneously using a particle swarm optimization algorithm. The prediction errors for biomass, glucose, and lactic acid concentrations were measured between 6.1 and 10.5%; the minimum error value was related to the prediction of biomass and the maximum one was related to the prediction of glucose using the model-based calibration approach. The model-based calibration approach and the classical approach showed similar results. In conclusion, the findings showed that a model-based calibration approach could be used to monitor the process state variables (i.e., biomass, glucose, and lactic acid) online in the fermentation process of a teff-based substrate inoculated with mixed strains of LPA6 and LCGG. However, glucose prediction showed a high error value.
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spelling pubmed-101430082023-04-29 Online Monitoring of the Growth of Probiotic Bacteria and Metabolites in the Fermentation of a Teff Substrate Using Model-Based Calibration of 2D Fluorescence Spectra Alemneh, Sendeku Takele Babor, Majharulislam Zettel, Viktoria von Wrochem, Almut Hitzmann, Bernd Microorganisms Article The demand for probiotic bacteria-fermented food products is increasing; however, the monitoring of the fermentation process is still challenging when using conventional approaches. A classical approach requires a large amount of offline data to calibrate a chemometric model using fluorescence spectra. Fluorescence spectra provide a wide range of online information during the process of cultivation, but they require a large amount of offline data (which involves laborious work) for the calibration procedure when using a classical approach. In this study, an alternative model-based calibration approach was used to predict biomass (the growth of Lactiplantibacillus plantarum A6 (LPA6) and Lacticaseibacillus rhamnosus GG (LCGG)), glucose, and lactic acid during the fermentation process of a teff-based substrate inoculated with mixed strains of LPA6 and LCGG. A classical approach was also applied and compared to the model-based calibration approach. In the model-based calibration approach, two-dimensional (2D) fluorescence spectra and offline substituted simulated data were used to generate a chemometric model. The optimum microbial specific growth rate and chemometric model parameters were obtained simultaneously using a particle swarm optimization algorithm. The prediction errors for biomass, glucose, and lactic acid concentrations were measured between 6.1 and 10.5%; the minimum error value was related to the prediction of biomass and the maximum one was related to the prediction of glucose using the model-based calibration approach. The model-based calibration approach and the classical approach showed similar results. In conclusion, the findings showed that a model-based calibration approach could be used to monitor the process state variables (i.e., biomass, glucose, and lactic acid) online in the fermentation process of a teff-based substrate inoculated with mixed strains of LPA6 and LCGG. However, glucose prediction showed a high error value. MDPI 2023-04-15 /pmc/articles/PMC10143008/ /pubmed/37110455 http://dx.doi.org/10.3390/microorganisms11041032 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alemneh, Sendeku Takele
Babor, Majharulislam
Zettel, Viktoria
von Wrochem, Almut
Hitzmann, Bernd
Online Monitoring of the Growth of Probiotic Bacteria and Metabolites in the Fermentation of a Teff Substrate Using Model-Based Calibration of 2D Fluorescence Spectra
title Online Monitoring of the Growth of Probiotic Bacteria and Metabolites in the Fermentation of a Teff Substrate Using Model-Based Calibration of 2D Fluorescence Spectra
title_full Online Monitoring of the Growth of Probiotic Bacteria and Metabolites in the Fermentation of a Teff Substrate Using Model-Based Calibration of 2D Fluorescence Spectra
title_fullStr Online Monitoring of the Growth of Probiotic Bacteria and Metabolites in the Fermentation of a Teff Substrate Using Model-Based Calibration of 2D Fluorescence Spectra
title_full_unstemmed Online Monitoring of the Growth of Probiotic Bacteria and Metabolites in the Fermentation of a Teff Substrate Using Model-Based Calibration of 2D Fluorescence Spectra
title_short Online Monitoring of the Growth of Probiotic Bacteria and Metabolites in the Fermentation of a Teff Substrate Using Model-Based Calibration of 2D Fluorescence Spectra
title_sort online monitoring of the growth of probiotic bacteria and metabolites in the fermentation of a teff substrate using model-based calibration of 2d fluorescence spectra
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143008/
https://www.ncbi.nlm.nih.gov/pubmed/37110455
http://dx.doi.org/10.3390/microorganisms11041032
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