Single-Stranded DNA-Binding Proteins Mediate DSB Repair and Effectively Improve CRISPR/Cas9 Genome Editing in Escherichia coli and Pseudomonas

Single-stranded DNA-binding proteins (SSBs) are essential for all living organisms. Whether SSBs can repair DNA double-strand breaks (DSBs) and improve the efficiency of CRISPR/Cas9-mediated genome editing has not been determined. Here, based on a pCas/pTargetF system, we constructed pCas-SSB and pC...

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Detalles Bibliográficos
Autores principales: Chai, Ran, Zhang, Qi, Wu, Jie, Shi, Ziwen, Li, Yanan, Gao, Yuqian, Qi, Yuancheng, Qiu, Liyou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143710/
https://www.ncbi.nlm.nih.gov/pubmed/37110272
http://dx.doi.org/10.3390/microorganisms11040850

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143710/
https://www.ncbi.nlm.nih.gov/pubmed/37110272
http://dx.doi.org/10.3390/microorganisms11040850

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