Activation of Insulin Gene Expression via Transfection of a CRISPR/dCas9a System Using Magnetic Peptide-Imprinted Nanoparticles

A CRISPRa transcription activation system was used to upregulate insulin expression in HEK293T cells. To increase the delivery of the targeted CRISPR/dCas9a, magnetic chitosan nanoparticles, imprinted with a peptide from the Cas9 protein, were developed, characterized, and then bound to dCas9a that...

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Detalles Bibliográficos
Autores principales: Lee, Mei-Hwa, Thomas, James L., Lin, Chien-Yu, Li, Yi-Chen Ethan, Lin, Hung-Yin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144049/
https://www.ncbi.nlm.nih.gov/pubmed/37111797
http://dx.doi.org/10.3390/pharmaceutics15041311

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144049/
https://www.ncbi.nlm.nih.gov/pubmed/37111797
http://dx.doi.org/10.3390/pharmaceutics15041311

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