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Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency
Doxycycline (DX) is a well-established and broad-spectrum antimicrobial drug. However, DX has drawbacks, such as physicochemical instability in aqueous media and bacterial resistance. The inclusion of drugs in cyclodextrin complexes and their loading into nanocarriers can overcome these limitations....
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144562/ https://www.ncbi.nlm.nih.gov/pubmed/37111770 http://dx.doi.org/10.3390/pharmaceutics15041285 |
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author | Carvalho Feitosa, Renata Souza Ribeiro Costa, Juliana van Vliet Lima, Marcelo Sawa Akioka Ishikawa, Elina Cogo Müller, Karina Bonin Okasaki, Fernando Sabadini, Edvaldo Garnero, Claudia Longhi, Marcela Raquel Lavayen, Vladimir da Silva-Júnior, Arnóbio Antônio Oliveira-Nascimento, Laura |
author_facet | Carvalho Feitosa, Renata Souza Ribeiro Costa, Juliana van Vliet Lima, Marcelo Sawa Akioka Ishikawa, Elina Cogo Müller, Karina Bonin Okasaki, Fernando Sabadini, Edvaldo Garnero, Claudia Longhi, Marcela Raquel Lavayen, Vladimir da Silva-Júnior, Arnóbio Antônio Oliveira-Nascimento, Laura |
author_sort | Carvalho Feitosa, Renata |
collection | PubMed |
description | Doxycycline (DX) is a well-established and broad-spectrum antimicrobial drug. However, DX has drawbacks, such as physicochemical instability in aqueous media and bacterial resistance. The inclusion of drugs in cyclodextrin complexes and their loading into nanocarriers can overcome these limitations. Thus, we studied the DX/sulfobutylether-β-CD (SBE-β-CD) inclusion complex for the first time and used it to reticulate chitosan. The resulting particles were evaluated by their physicochemical characteristics and antibacterial activity. DX/SBE-β-CD complexes were characterized by nuclear magnetic resonance, infrared spectroscopy, thermal analysis, X-ray diffraction, and scanning electron microscopy (SEM), whereas DX-loaded nanoparticles were characterized by dynamic light scattering, SEM, and drug content. The partial inclusion of the DX molecule in CD happened in a 1:1 proportion and brought increased stability to solid DX upon thermal degradation. Chitosan-complex nanoparticles measured approximately 200 nm, with a narrow polydispersity and particles with sufficient drug encapsulation for microbiological studies. Both formulations preserved the antimicrobial activity of DX against Staphylococcus aureus, whereas DX/SBE-β-CD inclusion complexes were also active against Klebsiella pneumoniae, indicating the potential use of these formulations as drug delivery systems to treat local infections. |
format | Online Article Text |
id | pubmed-10144562 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101445622023-04-29 Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency Carvalho Feitosa, Renata Souza Ribeiro Costa, Juliana van Vliet Lima, Marcelo Sawa Akioka Ishikawa, Elina Cogo Müller, Karina Bonin Okasaki, Fernando Sabadini, Edvaldo Garnero, Claudia Longhi, Marcela Raquel Lavayen, Vladimir da Silva-Júnior, Arnóbio Antônio Oliveira-Nascimento, Laura Pharmaceutics Article Doxycycline (DX) is a well-established and broad-spectrum antimicrobial drug. However, DX has drawbacks, such as physicochemical instability in aqueous media and bacterial resistance. The inclusion of drugs in cyclodextrin complexes and their loading into nanocarriers can overcome these limitations. Thus, we studied the DX/sulfobutylether-β-CD (SBE-β-CD) inclusion complex for the first time and used it to reticulate chitosan. The resulting particles were evaluated by their physicochemical characteristics and antibacterial activity. DX/SBE-β-CD complexes were characterized by nuclear magnetic resonance, infrared spectroscopy, thermal analysis, X-ray diffraction, and scanning electron microscopy (SEM), whereas DX-loaded nanoparticles were characterized by dynamic light scattering, SEM, and drug content. The partial inclusion of the DX molecule in CD happened in a 1:1 proportion and brought increased stability to solid DX upon thermal degradation. Chitosan-complex nanoparticles measured approximately 200 nm, with a narrow polydispersity and particles with sufficient drug encapsulation for microbiological studies. Both formulations preserved the antimicrobial activity of DX against Staphylococcus aureus, whereas DX/SBE-β-CD inclusion complexes were also active against Klebsiella pneumoniae, indicating the potential use of these formulations as drug delivery systems to treat local infections. MDPI 2023-04-19 /pmc/articles/PMC10144562/ /pubmed/37111770 http://dx.doi.org/10.3390/pharmaceutics15041285 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Carvalho Feitosa, Renata Souza Ribeiro Costa, Juliana van Vliet Lima, Marcelo Sawa Akioka Ishikawa, Elina Cogo Müller, Karina Bonin Okasaki, Fernando Sabadini, Edvaldo Garnero, Claudia Longhi, Marcela Raquel Lavayen, Vladimir da Silva-Júnior, Arnóbio Antônio Oliveira-Nascimento, Laura Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency |
title | Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency |
title_full | Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency |
title_fullStr | Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency |
title_full_unstemmed | Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency |
title_short | Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency |
title_sort | supramolecular arrangement of doxycycline with sulfobutylether-β-cyclodextrin: impact on nanostructuration with chitosan, drug degradation and antimicrobial potency |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144562/ https://www.ncbi.nlm.nih.gov/pubmed/37111770 http://dx.doi.org/10.3390/pharmaceutics15041285 |
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