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Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency

Doxycycline (DX) is a well-established and broad-spectrum antimicrobial drug. However, DX has drawbacks, such as physicochemical instability in aqueous media and bacterial resistance. The inclusion of drugs in cyclodextrin complexes and their loading into nanocarriers can overcome these limitations....

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Autores principales: Carvalho Feitosa, Renata, Souza Ribeiro Costa, Juliana, van Vliet Lima, Marcelo, Sawa Akioka Ishikawa, Elina, Cogo Müller, Karina, Bonin Okasaki, Fernando, Sabadini, Edvaldo, Garnero, Claudia, Longhi, Marcela Raquel, Lavayen, Vladimir, da Silva-Júnior, Arnóbio Antônio, Oliveira-Nascimento, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144562/
https://www.ncbi.nlm.nih.gov/pubmed/37111770
http://dx.doi.org/10.3390/pharmaceutics15041285
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author Carvalho Feitosa, Renata
Souza Ribeiro Costa, Juliana
van Vliet Lima, Marcelo
Sawa Akioka Ishikawa, Elina
Cogo Müller, Karina
Bonin Okasaki, Fernando
Sabadini, Edvaldo
Garnero, Claudia
Longhi, Marcela Raquel
Lavayen, Vladimir
da Silva-Júnior, Arnóbio Antônio
Oliveira-Nascimento, Laura
author_facet Carvalho Feitosa, Renata
Souza Ribeiro Costa, Juliana
van Vliet Lima, Marcelo
Sawa Akioka Ishikawa, Elina
Cogo Müller, Karina
Bonin Okasaki, Fernando
Sabadini, Edvaldo
Garnero, Claudia
Longhi, Marcela Raquel
Lavayen, Vladimir
da Silva-Júnior, Arnóbio Antônio
Oliveira-Nascimento, Laura
author_sort Carvalho Feitosa, Renata
collection PubMed
description Doxycycline (DX) is a well-established and broad-spectrum antimicrobial drug. However, DX has drawbacks, such as physicochemical instability in aqueous media and bacterial resistance. The inclusion of drugs in cyclodextrin complexes and their loading into nanocarriers can overcome these limitations. Thus, we studied the DX/sulfobutylether-β-CD (SBE-β-CD) inclusion complex for the first time and used it to reticulate chitosan. The resulting particles were evaluated by their physicochemical characteristics and antibacterial activity. DX/SBE-β-CD complexes were characterized by nuclear magnetic resonance, infrared spectroscopy, thermal analysis, X-ray diffraction, and scanning electron microscopy (SEM), whereas DX-loaded nanoparticles were characterized by dynamic light scattering, SEM, and drug content. The partial inclusion of the DX molecule in CD happened in a 1:1 proportion and brought increased stability to solid DX upon thermal degradation. Chitosan-complex nanoparticles measured approximately 200 nm, with a narrow polydispersity and particles with sufficient drug encapsulation for microbiological studies. Both formulations preserved the antimicrobial activity of DX against Staphylococcus aureus, whereas DX/SBE-β-CD inclusion complexes were also active against Klebsiella pneumoniae, indicating the potential use of these formulations as drug delivery systems to treat local infections.
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spelling pubmed-101445622023-04-29 Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency Carvalho Feitosa, Renata Souza Ribeiro Costa, Juliana van Vliet Lima, Marcelo Sawa Akioka Ishikawa, Elina Cogo Müller, Karina Bonin Okasaki, Fernando Sabadini, Edvaldo Garnero, Claudia Longhi, Marcela Raquel Lavayen, Vladimir da Silva-Júnior, Arnóbio Antônio Oliveira-Nascimento, Laura Pharmaceutics Article Doxycycline (DX) is a well-established and broad-spectrum antimicrobial drug. However, DX has drawbacks, such as physicochemical instability in aqueous media and bacterial resistance. The inclusion of drugs in cyclodextrin complexes and their loading into nanocarriers can overcome these limitations. Thus, we studied the DX/sulfobutylether-β-CD (SBE-β-CD) inclusion complex for the first time and used it to reticulate chitosan. The resulting particles were evaluated by their physicochemical characteristics and antibacterial activity. DX/SBE-β-CD complexes were characterized by nuclear magnetic resonance, infrared spectroscopy, thermal analysis, X-ray diffraction, and scanning electron microscopy (SEM), whereas DX-loaded nanoparticles were characterized by dynamic light scattering, SEM, and drug content. The partial inclusion of the DX molecule in CD happened in a 1:1 proportion and brought increased stability to solid DX upon thermal degradation. Chitosan-complex nanoparticles measured approximately 200 nm, with a narrow polydispersity and particles with sufficient drug encapsulation for microbiological studies. Both formulations preserved the antimicrobial activity of DX against Staphylococcus aureus, whereas DX/SBE-β-CD inclusion complexes were also active against Klebsiella pneumoniae, indicating the potential use of these formulations as drug delivery systems to treat local infections. MDPI 2023-04-19 /pmc/articles/PMC10144562/ /pubmed/37111770 http://dx.doi.org/10.3390/pharmaceutics15041285 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Carvalho Feitosa, Renata
Souza Ribeiro Costa, Juliana
van Vliet Lima, Marcelo
Sawa Akioka Ishikawa, Elina
Cogo Müller, Karina
Bonin Okasaki, Fernando
Sabadini, Edvaldo
Garnero, Claudia
Longhi, Marcela Raquel
Lavayen, Vladimir
da Silva-Júnior, Arnóbio Antônio
Oliveira-Nascimento, Laura
Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency
title Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency
title_full Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency
title_fullStr Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency
title_full_unstemmed Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency
title_short Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency
title_sort supramolecular arrangement of doxycycline with sulfobutylether-β-cyclodextrin: impact on nanostructuration with chitosan, drug degradation and antimicrobial potency
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144562/
https://www.ncbi.nlm.nih.gov/pubmed/37111770
http://dx.doi.org/10.3390/pharmaceutics15041285
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