An optimized protocol to isolate quiescent washed platelets from human whole blood and generate platelet releasate under clinical conditions

The contents of the platelet releasate (PR) play significant roles in hemostasis, inflammation, and pathologic sequelae. Careful platelet isolation to ensure quiescence and subsequent activation is key to the successful generation of PR. Here, we describe steps to isolate and aggregate quiescent was...

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Autores principales: Weiss, Luisa, MacLeod, Hayley, Comer, Shane P., Cullivan, Sarah, Szklanna, Paulina B., Ní Áinle, Fionnuala, Kevane, Barry, Maguire, Patricia B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10148026/
https://www.ncbi.nlm.nih.gov/pubmed/37074908
http://dx.doi.org/10.1016/j.xpro.2023.102150
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author Weiss, Luisa
MacLeod, Hayley
Comer, Shane P.
Cullivan, Sarah
Szklanna, Paulina B.
Ní Áinle, Fionnuala
Kevane, Barry
Maguire, Patricia B.
author_facet Weiss, Luisa
MacLeod, Hayley
Comer, Shane P.
Cullivan, Sarah
Szklanna, Paulina B.
Ní Áinle, Fionnuala
Kevane, Barry
Maguire, Patricia B.
author_sort Weiss, Luisa
collection PubMed
description The contents of the platelet releasate (PR) play significant roles in hemostasis, inflammation, and pathologic sequelae. Careful platelet isolation to ensure quiescence and subsequent activation is key to the successful generation of PR. Here, we describe steps to isolate and aggregate quiescent washed platelets from whole blood of a clinical patient cohort. We then detail the generation of PR from isolated human washed platelets under clinical conditions. This protocol allows the investigation of platelet cargoes released through various activation pathways.
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spelling pubmed-101480262023-04-30 An optimized protocol to isolate quiescent washed platelets from human whole blood and generate platelet releasate under clinical conditions Weiss, Luisa MacLeod, Hayley Comer, Shane P. Cullivan, Sarah Szklanna, Paulina B. Ní Áinle, Fionnuala Kevane, Barry Maguire, Patricia B. STAR Protoc Protocol The contents of the platelet releasate (PR) play significant roles in hemostasis, inflammation, and pathologic sequelae. Careful platelet isolation to ensure quiescence and subsequent activation is key to the successful generation of PR. Here, we describe steps to isolate and aggregate quiescent washed platelets from whole blood of a clinical patient cohort. We then detail the generation of PR from isolated human washed platelets under clinical conditions. This protocol allows the investigation of platelet cargoes released through various activation pathways. Elsevier 2023-04-18 /pmc/articles/PMC10148026/ /pubmed/37074908 http://dx.doi.org/10.1016/j.xpro.2023.102150 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Weiss, Luisa
MacLeod, Hayley
Comer, Shane P.
Cullivan, Sarah
Szklanna, Paulina B.
Ní Áinle, Fionnuala
Kevane, Barry
Maguire, Patricia B.
An optimized protocol to isolate quiescent washed platelets from human whole blood and generate platelet releasate under clinical conditions
title An optimized protocol to isolate quiescent washed platelets from human whole blood and generate platelet releasate under clinical conditions
title_full An optimized protocol to isolate quiescent washed platelets from human whole blood and generate platelet releasate under clinical conditions
title_fullStr An optimized protocol to isolate quiescent washed platelets from human whole blood and generate platelet releasate under clinical conditions
title_full_unstemmed An optimized protocol to isolate quiescent washed platelets from human whole blood and generate platelet releasate under clinical conditions
title_short An optimized protocol to isolate quiescent washed platelets from human whole blood and generate platelet releasate under clinical conditions
title_sort optimized protocol to isolate quiescent washed platelets from human whole blood and generate platelet releasate under clinical conditions
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10148026/
https://www.ncbi.nlm.nih.gov/pubmed/37074908
http://dx.doi.org/10.1016/j.xpro.2023.102150
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