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Evaluation of DNA extraction methods and direct PCR in metabarcoding of mock and marine bacterial communities

Recent advances in new molecular biology methods and next-generation sequencing (NGS) technologies have revolutionized metabarcoding studies investigating complex microbial communities from various environments. The inevitable first step in sample preparation is DNA extraction which introduces its o...

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Detalles Bibliográficos
Autores principales: Stojan, Iva, Trumbić, Željka, Lepen Pleić, Ivana, Šantić, Danijela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10149847/
https://www.ncbi.nlm.nih.gov/pubmed/37138601
http://dx.doi.org/10.3389/fmicb.2023.1151907