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Mechanism of imidazole inhibition of a GH1 β‐glucosidase

Imidazole is largely employed in recombinant protein purification, including GH1 β‐glucosidases, but its effect on the enzyme activity is rarely taken into consideration. Computational docking suggested that imidazole interacts with residues forming the active site of the GH1 β‐glucosidase from Spod...

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Detalles Bibliográficos
Autores principales: Chagas, Rafael S., Otsuka, Felipe A. M., Pineda, Mario A. R., Salinas, Roberto K., Marana, Sandro R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10153361/
https://www.ncbi.nlm.nih.gov/pubmed/36906930
http://dx.doi.org/10.1002/2211-5463.13595