Ultrafast RNA extraction-free SARS-CoV-2 detection by direct RT-PCR using a rapid thermal cycling approach

The COVID19 pandemic has underlined the need for quick and high-throughput SARS-CoV-2 detection assays. Here we report the development of a direct RT-PCR detection method that can reliably detect SARS-CoV-2 gRNA in nasopharyngeal swab samples in under 27 minutes without needing nucleic acid extracti...

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Detalles Bibliográficos
Autores principales: Struijk, Robin, van den Ouden, Anton, Louwerse, Jeroen, Čurová, Katarína, Burggrave, Ronald, McNally, Brian, de Groot, Theun, Mulder, Bert, de Vos, Gert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). Published by Elsevier Inc. 2023
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154057/
https://www.ncbi.nlm.nih.gov/pubmed/37343400
http://dx.doi.org/10.1016/j.diagmicrobio.2023.115975

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154057/
https://www.ncbi.nlm.nih.gov/pubmed/37343400
http://dx.doi.org/10.1016/j.diagmicrobio.2023.115975

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