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Ultrafast RNA extraction-free SARS-CoV-2 detection by direct RT-PCR using a rapid thermal cycling approach
The COVID19 pandemic has underlined the need for quick and high-throughput SARS-CoV-2 detection assays. Here we report the development of a direct RT-PCR detection method that can reliably detect SARS-CoV-2 gRNA in nasopharyngeal swab samples in under 27 minutes without needing nucleic acid extracti...
Autores principales: | Struijk, Robin, van den Ouden, Anton, Louwerse, Jeroen, Čurová, Katarína, Burggrave, Ronald, McNally, Brian, de Groot, Theun, Mulder, Bert, de Vos, Gert |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Author(s). Published by Elsevier Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154057/ https://www.ncbi.nlm.nih.gov/pubmed/37343400 http://dx.doi.org/10.1016/j.diagmicrobio.2023.115975 |
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