Ligand-directed labeling of opioid receptors for covalent attachment of fluorophores or small-molecule probes

This protocol describes endogenous labeling of opioid receptors (ORs) using a ligand-directed reagent, naltrexamine-acylimidazole compounds (NAI-X). NAI acts by guiding and permanently tagging a small-molecule reporter (X)—such as fluorophores or biotin—to ORs. Here we detail syntheses and uses of N...

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Detalles Bibliográficos
Autores principales: Adoff, Hayden, Halls, Victoria S., Holland, Emily, Lobingier, Braden, Arttamangkul, Seksiri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154970/
https://www.ncbi.nlm.nih.gov/pubmed/37104091
http://dx.doi.org/10.1016/j.xpro.2023.102231

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154970/
https://www.ncbi.nlm.nih.gov/pubmed/37104091
http://dx.doi.org/10.1016/j.xpro.2023.102231

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