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Ligand-directed labeling of opioid receptors for covalent attachment of fluorophores or small-molecule probes
This protocol describes endogenous labeling of opioid receptors (ORs) using a ligand-directed reagent, naltrexamine-acylimidazole compounds (NAI-X). NAI acts by guiding and permanently tagging a small-molecule reporter (X)—such as fluorophores or biotin—to ORs. Here we detail syntheses and uses of N...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154970/ https://www.ncbi.nlm.nih.gov/pubmed/37104091 http://dx.doi.org/10.1016/j.xpro.2023.102231 |