Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice

Engineered microorganisms hold potential for disease diagnosis and treatment. Here, we present a protocol to engineer E. coli Nissle 1917 strain (iROBOT) using genome insertion and plasmid construction to diagnose, record, and ameliorate inflammatory bowel disease in mice. We describe steps for cons...

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Detalles Bibliográficos
Autores principales: Zou, Zhen-Ping, Fan, Yu-Hang, Du, Yue, Fang, Ting-Ting, Du, Wei, Zhou, Ying, Ye, Bang-Ce
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154985/
https://www.ncbi.nlm.nih.gov/pubmed/37115666
http://dx.doi.org/10.1016/j.xpro.2023.102254

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154985/
https://www.ncbi.nlm.nih.gov/pubmed/37115666
http://dx.doi.org/10.1016/j.xpro.2023.102254

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