DNA methylation protocol for analyzing cell-free DNA in the spent culture medium of human preimplantation embryos

Cell-free DNA (cfDNA) in spent embryo culture media (SECM) provides prospects for noninvasive preimplantation genetic testing. Here, we present a post-bisulfite-adapter-tagging (PBAT)-based whole-genome DNA methylation sequencing protocol (SECM-PBAT) for human SECM cfDNA analysis. We describe steps...

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Detalles Bibliográficos
Autores principales: Gao, Yuan, Chen, Yidong, Qiao, Jie, Huang, Jin, Wen, Lu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160802/
https://www.ncbi.nlm.nih.gov/pubmed/37086412
http://dx.doi.org/10.1016/j.xpro.2023.102247

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160802/
https://www.ncbi.nlm.nih.gov/pubmed/37086412
http://dx.doi.org/10.1016/j.xpro.2023.102247

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