Nanostructured 3D‐Printed Hybrid Scaffold Accelerates Bone Regeneration by Photointegrating Nanohydroxyapatite

Nanostructured biomaterials that replicate natural bone architecture are expected to facilitate bone regeneration. Here, nanohydroxyapatite (nHAp) with vinyl surface modification is acquired by silicon‐based coupling agent and photointegrated with methacrylic anhydride‐modified gelatin to manufactur...

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Autores principales: Tong, Lei, Pu, Xiaocong, Liu, Quanying, Li, Xing, Chen, Manyu, Wang, Peilei, Zou, Yaping, Lu, Gonggong, Liang, Jie, Fan, Yujiang, Zhang, Xingdong, Sun, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10161056/
https://www.ncbi.nlm.nih.gov/pubmed/36905235
http://dx.doi.org/10.1002/advs.202300038
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author Tong, Lei
Pu, Xiaocong
Liu, Quanying
Li, Xing
Chen, Manyu
Wang, Peilei
Zou, Yaping
Lu, Gonggong
Liang, Jie
Fan, Yujiang
Zhang, Xingdong
Sun, Yong
author_facet Tong, Lei
Pu, Xiaocong
Liu, Quanying
Li, Xing
Chen, Manyu
Wang, Peilei
Zou, Yaping
Lu, Gonggong
Liang, Jie
Fan, Yujiang
Zhang, Xingdong
Sun, Yong
author_sort Tong, Lei
collection PubMed
description Nanostructured biomaterials that replicate natural bone architecture are expected to facilitate bone regeneration. Here, nanohydroxyapatite (nHAp) with vinyl surface modification is acquired by silicon‐based coupling agent and photointegrated with methacrylic anhydride‐modified gelatin to manufacture a chemically integrated 3D‐printed hybrid bone scaffold (75.6 wt% solid content). This nanostructured procedure significantly increases its storage modulus by 19.43‐fold (79.2 kPa) to construct a more stable mechanical structure. Furthermore, biofunctional hydrogel with biomimetic extracellular matrix is anchored onto the filament of 3D‐printed hybrid scaffold (HGel‐g‐nHAp) by polyphenol‐mediated multiple chemical reactions, which contributes to initiate early osteogenesis and angiogenesis by recruiting endogenous stem cells in situ. Significant ectopic mineral deposition is also observed in subcutaneously implanted nude mice with storage modulus enhancement of 25.3‐fold after 30 days. Meanwhile, HGel‐g‐nHAp realizes substantial bone reconstruction in the rabbit cranial defect model, achieving 61.3% breaking load strength and 73.1% bone volume fractions in comparison to natural cranium 15 weeks after implantation. This optical integration strategy of vinyl modified nHAp provides a prospective structural design for regenerative 3D‐printed bone scaffold.
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spelling pubmed-101610562023-05-06 Nanostructured 3D‐Printed Hybrid Scaffold Accelerates Bone Regeneration by Photointegrating Nanohydroxyapatite Tong, Lei Pu, Xiaocong Liu, Quanying Li, Xing Chen, Manyu Wang, Peilei Zou, Yaping Lu, Gonggong Liang, Jie Fan, Yujiang Zhang, Xingdong Sun, Yong Adv Sci (Weinh) Research Articles Nanostructured biomaterials that replicate natural bone architecture are expected to facilitate bone regeneration. Here, nanohydroxyapatite (nHAp) with vinyl surface modification is acquired by silicon‐based coupling agent and photointegrated with methacrylic anhydride‐modified gelatin to manufacture a chemically integrated 3D‐printed hybrid bone scaffold (75.6 wt% solid content). This nanostructured procedure significantly increases its storage modulus by 19.43‐fold (79.2 kPa) to construct a more stable mechanical structure. Furthermore, biofunctional hydrogel with biomimetic extracellular matrix is anchored onto the filament of 3D‐printed hybrid scaffold (HGel‐g‐nHAp) by polyphenol‐mediated multiple chemical reactions, which contributes to initiate early osteogenesis and angiogenesis by recruiting endogenous stem cells in situ. Significant ectopic mineral deposition is also observed in subcutaneously implanted nude mice with storage modulus enhancement of 25.3‐fold after 30 days. Meanwhile, HGel‐g‐nHAp realizes substantial bone reconstruction in the rabbit cranial defect model, achieving 61.3% breaking load strength and 73.1% bone volume fractions in comparison to natural cranium 15 weeks after implantation. This optical integration strategy of vinyl modified nHAp provides a prospective structural design for regenerative 3D‐printed bone scaffold. John Wiley and Sons Inc. 2023-03-11 /pmc/articles/PMC10161056/ /pubmed/36905235 http://dx.doi.org/10.1002/advs.202300038 Text en © 2023 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Tong, Lei
Pu, Xiaocong
Liu, Quanying
Li, Xing
Chen, Manyu
Wang, Peilei
Zou, Yaping
Lu, Gonggong
Liang, Jie
Fan, Yujiang
Zhang, Xingdong
Sun, Yong
Nanostructured 3D‐Printed Hybrid Scaffold Accelerates Bone Regeneration by Photointegrating Nanohydroxyapatite
title Nanostructured 3D‐Printed Hybrid Scaffold Accelerates Bone Regeneration by Photointegrating Nanohydroxyapatite
title_full Nanostructured 3D‐Printed Hybrid Scaffold Accelerates Bone Regeneration by Photointegrating Nanohydroxyapatite
title_fullStr Nanostructured 3D‐Printed Hybrid Scaffold Accelerates Bone Regeneration by Photointegrating Nanohydroxyapatite
title_full_unstemmed Nanostructured 3D‐Printed Hybrid Scaffold Accelerates Bone Regeneration by Photointegrating Nanohydroxyapatite
title_short Nanostructured 3D‐Printed Hybrid Scaffold Accelerates Bone Regeneration by Photointegrating Nanohydroxyapatite
title_sort nanostructured 3d‐printed hybrid scaffold accelerates bone regeneration by photointegrating nanohydroxyapatite
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10161056/
https://www.ncbi.nlm.nih.gov/pubmed/36905235
http://dx.doi.org/10.1002/advs.202300038
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