High-throughput and high-accuracy single-cell RNA isoform analysis using PacBio circular consensus sequencing

Although long-read single-cell RNA isoform sequencing (scISO-Seq) can reveal alternative RNA splicing in individual cells, it suffers from a low read throughput. Here, we introduce HIT-scISOseq, a method that removes most artifact cDNAs and concatenates multiple cDNAs for PacBio circular consensus s...

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Detalles Bibliográficos
Autores principales: Shi, Zhuo-Xing, Chen, Zhi-Chao, Zhong, Jia-Yong, Hu, Kun-Hua, Zheng, Ying-Feng, Chen, Ying, Xie, Shang-Qian, Bo, Xiao-Chen, Luo, Feng, Tang, Chong, Xiao, Chuan-Le, Liu, Yi-Zhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10164132/
https://www.ncbi.nlm.nih.gov/pubmed/37149708
http://dx.doi.org/10.1038/s41467-023-38324-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10164132/
https://www.ncbi.nlm.nih.gov/pubmed/37149708
http://dx.doi.org/10.1038/s41467-023-38324-9

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