DNA rehybridization drives product release from Cas9 ribonucleoprotein to enable multiple-turnover cleavage

The RNA-guided Cas9 endonuclease from Staphylococcus aureus (SauCas9) can catalyze multiple-turnover reactions whereas Cas9 from Streptococcus pyogenes (SpyCas9) is a single-turnover enzyme. Here we dissect the mechanism of multiple-turnover catalysis by SauCas9 and elucidate its molecular basis. We...

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Detalles Bibliográficos
Autores principales: Pan, Juan, Mabuchi, Megumu, Robb, Gregory Brett
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10164561/
https://www.ncbi.nlm.nih.gov/pubmed/37014013
http://dx.doi.org/10.1093/nar/gkad233

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10164561/
https://www.ncbi.nlm.nih.gov/pubmed/37014013
http://dx.doi.org/10.1093/nar/gkad233

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