High-throughput methodology to identify CRISPR-generated Danio rerio mutants using fragment analysis with unmodified PCR products

Targeted mutagenesis in zebrafish, fruit flies, and C. elegans has been significantly improved over the years through CRISPR technology. CRISPR enables researchers to efficiently examine cellular pathways by inducing small, targeted mutations in vivo. Though these mutations are commonly random inser...

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Detalles Bibliográficos
Autores principales: Colijn, Sarah, Yin, Ying, Stratman, Amber N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10164608/
https://www.ncbi.nlm.nih.gov/pubmed/35149003
http://dx.doi.org/10.1016/j.ydbio.2022.02.003

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10164608/
https://www.ncbi.nlm.nih.gov/pubmed/35149003
http://dx.doi.org/10.1016/j.ydbio.2022.02.003

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