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High-throughput methodology to identify CRISPR-generated Danio rerio mutants using fragment analysis with unmodified PCR products
Targeted mutagenesis in zebrafish, fruit flies, and C. elegans has been significantly improved over the years through CRISPR technology. CRISPR enables researchers to efficiently examine cellular pathways by inducing small, targeted mutations in vivo. Though these mutations are commonly random inser...
Autores principales: | Colijn, Sarah, Yin, Ying, Stratman, Amber N. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10164608/ https://www.ncbi.nlm.nih.gov/pubmed/35149003 http://dx.doi.org/10.1016/j.ydbio.2022.02.003 |
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