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Exploring linker's sequence diversity to fuse carotene cyclase and hydroxylase for zeaxanthin biosynthesis

Fusion of catalytic domains can accelerate cascade reactions by bringing enzymes in close proximity. However, the design of a protein fusion and the choice of a linker are often challenging and lack of guidance. To determine the impact of linker parameters on fusion proteins, a library of linkers fe...

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Detalles Bibliográficos
Autores principales: Bouin, Aurélie, Zhang, Congqiang, Lindley, Nic D., Truan, Gilles, Lautier, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165439/
https://www.ncbi.nlm.nih.gov/pubmed/37168436
http://dx.doi.org/10.1016/j.mec.2023.e00222