A protocol to study three-dimensional genome structure in individual mutant preimplantation mouse embryos

Hi-C studies the three-dimensional structure of the genome by detecting genome-wide chromatin regions that are in spatial proximity within the nucleus. We developed single-blastocyst Hi-C in mutant mouse embryos to genotype them on sequence. We describe steps for embryo fixation and nuclei permeabil...

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Detalles Bibliográficos
Autores principales: Gimenez-Llorente, Daniel, Portela, Marta, Losada, Ana, Manzanares, Miguel, Andreu, Maria Jose
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165443/
https://www.ncbi.nlm.nih.gov/pubmed/37126445
http://dx.doi.org/10.1016/j.xpro.2023.102267

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165443/
https://www.ncbi.nlm.nih.gov/pubmed/37126445
http://dx.doi.org/10.1016/j.xpro.2023.102267

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