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Mechanical isolation of neonatal and adult mouse dura leukocytes for flow cytometry analysis

The meninges, consisting of the pia, arachnoid, and dura layers, provide immunosurveillance of the central nervous system with both innate and adaptive immune cells. Here we present an optimized protocol for isolating dura leukocytes from neonatal and adult mice. We describe steps for harvesting the...

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Detalles Bibliográficos
Autores principales: Niu, Chunxiao, Jiang, Hui, Wu, Mengyao, Zou, Tao, Wu, Xian, Lu, Yuchen, Deng, Lijiao, Guo, Tingting, Si, Chuan-Yimu, Zhang, Jiyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165444/
https://www.ncbi.nlm.nih.gov/pubmed/37126441
http://dx.doi.org/10.1016/j.xpro.2023.102272