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Mechanical isolation of neonatal and adult mouse dura leukocytes for flow cytometry analysis
The meninges, consisting of the pia, arachnoid, and dura layers, provide immunosurveillance of the central nervous system with both innate and adaptive immune cells. Here we present an optimized protocol for isolating dura leukocytes from neonatal and adult mice. We describe steps for harvesting the...
| Autores principales: | , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165444/ https://www.ncbi.nlm.nih.gov/pubmed/37126441 http://dx.doi.org/10.1016/j.xpro.2023.102272 |