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Calcium measurements in enzymatically dissociated or mechanically microdissected mouse primary skeletal muscle fibers
Here, we provide a protocol for isolation of mouse primary skeletal muscle fibers using two alternative approaches—enzymatic dissociation or mechanical microdissection. We describe the procedures for surgical removal of muscle of interest and isolation of intact single-muscle fibers by either collag...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165447/ https://www.ncbi.nlm.nih.gov/pubmed/37126446 http://dx.doi.org/10.1016/j.xpro.2023.102260 |