Calcium measurements in enzymatically dissociated or mechanically microdissected mouse primary skeletal muscle fibers

Here, we provide a protocol for isolation of mouse primary skeletal muscle fibers using two alternative approaches—enzymatic dissociation or mechanical microdissection. We describe the procedures for surgical removal of muscle of interest and isolation of intact single-muscle fibers by either collag...

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Detalles Bibliográficos
Autores principales: Youhanna, Sonia, Bruton, Joseph, Jardemark, Kent, Westerblad, Håkan, Lauschke, Volker M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165447/
https://www.ncbi.nlm.nih.gov/pubmed/37126446
http://dx.doi.org/10.1016/j.xpro.2023.102260

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