A simple, dual direct expression plasmid system in prokaryotic and mammalian cells

We introduce a simple, dual direct cloning plasmid system (pgMAX-II) for gene expression analysis in both prokaryotic (Escherichia coli) and mammalian cells. This system, which uses a prokaryotic expression unit adapted from the pgMAX system and a mammalian promoter, is effective for subcloning usin...

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Detalles Bibliográficos
Autores principales: Murakami, Manabu, Murakami, Agnieszka M, Yonekura, Manabu, Miyoshi, Ichiro, Itagaki, Shirou, Niwa, Yasutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Biological, Health, and Medical Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165797/
https://www.ncbi.nlm.nih.gov/pubmed/37168669
http://dx.doi.org/10.1093/pnasnexus/pgad139

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165797/
https://www.ncbi.nlm.nih.gov/pubmed/37168669
http://dx.doi.org/10.1093/pnasnexus/pgad139

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