A simple, dual direct expression plasmid system in prokaryotic and mammalian cells

We introduce a simple, dual direct cloning plasmid system (pgMAX-II) for gene expression analysis in both prokaryotic (Escherichia coli) and mammalian cells. This system, which uses a prokaryotic expression unit adapted from the pgMAX system and a mammalian promoter, is effective for subcloning usin...

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Autores principales: Murakami, Manabu, Murakami, Agnieszka M, Yonekura, Manabu, Miyoshi, Ichiro, Itagaki, Shirou, Niwa, Yasutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Biological, Health, and Medical Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165797/
https://www.ncbi.nlm.nih.gov/pubmed/37168669
http://dx.doi.org/10.1093/pnasnexus/pgad139
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author Murakami, Manabu
Murakami, Agnieszka M
Yonekura, Manabu
Miyoshi, Ichiro
Itagaki, Shirou
Niwa, Yasutaka
author_facet Murakami, Manabu
Murakami, Agnieszka M
Yonekura, Manabu
Miyoshi, Ichiro
Itagaki, Shirou
Niwa, Yasutaka
author_sort Murakami, Manabu
collection PubMed
description We introduce a simple, dual direct cloning plasmid system (pgMAX-II) for gene expression analysis in both prokaryotic (Escherichia coli) and mammalian cells. This system, which uses a prokaryotic expression unit adapted from the pgMAX system and a mammalian promoter, is effective for subcloning using the DNA topoisomerase II toxin CcdB. Given that molecular biological cloning systems broadly rely on E. coli for rapid growth, the proposed concept may have wide applicability beyond mammalian cells.
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spelling pubmed-101657972023-05-09 A simple, dual direct expression plasmid system in prokaryotic and mammalian cells Murakami, Manabu Murakami, Agnieszka M Yonekura, Manabu Miyoshi, Ichiro Itagaki, Shirou Niwa, Yasutaka PNAS Nexus Biological, Health, and Medical Sciences We introduce a simple, dual direct cloning plasmid system (pgMAX-II) for gene expression analysis in both prokaryotic (Escherichia coli) and mammalian cells. This system, which uses a prokaryotic expression unit adapted from the pgMAX system and a mammalian promoter, is effective for subcloning using the DNA topoisomerase II toxin CcdB. Given that molecular biological cloning systems broadly rely on E. coli for rapid growth, the proposed concept may have wide applicability beyond mammalian cells. Oxford University Press 2023-04-18 /pmc/articles/PMC10165797/ /pubmed/37168669 http://dx.doi.org/10.1093/pnasnexus/pgad139 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of National Academy of Sciences. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Biological, Health, and Medical Sciences
Murakami, Manabu
Murakami, Agnieszka M
Yonekura, Manabu
Miyoshi, Ichiro
Itagaki, Shirou
Niwa, Yasutaka
A simple, dual direct expression plasmid system in prokaryotic and mammalian cells
title A simple, dual direct expression plasmid system in prokaryotic and mammalian cells
title_full A simple, dual direct expression plasmid system in prokaryotic and mammalian cells
title_fullStr A simple, dual direct expression plasmid system in prokaryotic and mammalian cells
title_full_unstemmed A simple, dual direct expression plasmid system in prokaryotic and mammalian cells
title_short A simple, dual direct expression plasmid system in prokaryotic and mammalian cells
title_sort simple, dual direct expression plasmid system in prokaryotic and mammalian cells
topic Biological, Health, and Medical Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165797/
https://www.ncbi.nlm.nih.gov/pubmed/37168669
http://dx.doi.org/10.1093/pnasnexus/pgad139
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