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Light-responsive transcription factor PpWRKY44 induces anthocyanin accumulation by regulating PpMYB10 expression in pear

Anthocyanins are a valuable source of antioxidants in the human diet and contribute to fruit coloration. In red-skinned pears, anthocyanin biosynthesis can be induced by light, in which the MYB–bHLH–WDR complex plays a critically important role in transcriptional regulation. However, knowledge of WR...

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Autores principales: Alabd, Ahmed, Ahmad, Mudassar, Zhang, Xiao, Gao, Yuhao, Peng, Lin, Zhang, Lu, Ni, Junbei, Bai, Songling, Teng, Yuanwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10167416/
https://www.ncbi.nlm.nih.gov/pubmed/37180030
http://dx.doi.org/10.1093/hr/uhac199
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author Alabd, Ahmed
Ahmad, Mudassar
Zhang, Xiao
Gao, Yuhao
Peng, Lin
Zhang, Lu
Ni, Junbei
Bai, Songling
Teng, Yuanwen
author_facet Alabd, Ahmed
Ahmad, Mudassar
Zhang, Xiao
Gao, Yuhao
Peng, Lin
Zhang, Lu
Ni, Junbei
Bai, Songling
Teng, Yuanwen
author_sort Alabd, Ahmed
collection PubMed
description Anthocyanins are a valuable source of antioxidants in the human diet and contribute to fruit coloration. In red-skinned pears, anthocyanin biosynthesis can be induced by light, in which the MYB–bHLH–WDR complex plays a critically important role in transcriptional regulation. However, knowledge of WRKY-mediated transcriptional regulation of light-induced anthocyanin biosynthesis is scarce in red pears. This work identified and functionally characterized a light-inducing WRKY transcription factor, PpWRKY44, in pear. Functional analysis based on overexpressed pear calli showed that PpWRKY44 promoted anthocyanin accumulation. Also, transiently overexpressed PpWRKY44 in pear leaves and fruit peels significantly enhanced the accumulation of anthocyanin, whereas silencing PpWRKY44 in pear fruit peels impaired induction of the accumulation of anthocyanin by light. By chromatin immunoprecipitation and electrophoretic mobility shift assay coupled to a quantitative polymerase chain reaction, we found that PpWRKY44 bound in vivo and in vitro to the PpMYB10 promoter, revealing it as a direct downstream target gene. Moreover, PpWRKY44 was activated by PpBBX18, a light signal transduction pathway component. Our results explained the mechanism mediating the impacts of PpWRKY44 on the transcriptional regulation of anthocyanin accumulation, with potential implications for fine-tuning the fruit peel coloration triggered by light in red pears.
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spelling pubmed-101674162023-05-10 Light-responsive transcription factor PpWRKY44 induces anthocyanin accumulation by regulating PpMYB10 expression in pear Alabd, Ahmed Ahmad, Mudassar Zhang, Xiao Gao, Yuhao Peng, Lin Zhang, Lu Ni, Junbei Bai, Songling Teng, Yuanwen Hortic Res Article Anthocyanins are a valuable source of antioxidants in the human diet and contribute to fruit coloration. In red-skinned pears, anthocyanin biosynthesis can be induced by light, in which the MYB–bHLH–WDR complex plays a critically important role in transcriptional regulation. However, knowledge of WRKY-mediated transcriptional regulation of light-induced anthocyanin biosynthesis is scarce in red pears. This work identified and functionally characterized a light-inducing WRKY transcription factor, PpWRKY44, in pear. Functional analysis based on overexpressed pear calli showed that PpWRKY44 promoted anthocyanin accumulation. Also, transiently overexpressed PpWRKY44 in pear leaves and fruit peels significantly enhanced the accumulation of anthocyanin, whereas silencing PpWRKY44 in pear fruit peels impaired induction of the accumulation of anthocyanin by light. By chromatin immunoprecipitation and electrophoretic mobility shift assay coupled to a quantitative polymerase chain reaction, we found that PpWRKY44 bound in vivo and in vitro to the PpMYB10 promoter, revealing it as a direct downstream target gene. Moreover, PpWRKY44 was activated by PpBBX18, a light signal transduction pathway component. Our results explained the mechanism mediating the impacts of PpWRKY44 on the transcriptional regulation of anthocyanin accumulation, with potential implications for fine-tuning the fruit peel coloration triggered by light in red pears. Oxford University Press 2022-09-06 /pmc/articles/PMC10167416/ /pubmed/37180030 http://dx.doi.org/10.1093/hr/uhac199 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Nanjing Agricultural University. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Alabd, Ahmed
Ahmad, Mudassar
Zhang, Xiao
Gao, Yuhao
Peng, Lin
Zhang, Lu
Ni, Junbei
Bai, Songling
Teng, Yuanwen
Light-responsive transcription factor PpWRKY44 induces anthocyanin accumulation by regulating PpMYB10 expression in pear
title Light-responsive transcription factor PpWRKY44 induces anthocyanin accumulation by regulating PpMYB10 expression in pear
title_full Light-responsive transcription factor PpWRKY44 induces anthocyanin accumulation by regulating PpMYB10 expression in pear
title_fullStr Light-responsive transcription factor PpWRKY44 induces anthocyanin accumulation by regulating PpMYB10 expression in pear
title_full_unstemmed Light-responsive transcription factor PpWRKY44 induces anthocyanin accumulation by regulating PpMYB10 expression in pear
title_short Light-responsive transcription factor PpWRKY44 induces anthocyanin accumulation by regulating PpMYB10 expression in pear
title_sort light-responsive transcription factor ppwrky44 induces anthocyanin accumulation by regulating ppmyb10 expression in pear
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10167416/
https://www.ncbi.nlm.nih.gov/pubmed/37180030
http://dx.doi.org/10.1093/hr/uhac199
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