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Wide-genome selection of lactic acid bacteria harboring genes that promote the elimination of antinutritional factors

Anti-nutritional factors (ANFs) substances in plant products, such as indigestible non-starchy polysaccharides (α-galactooligosaccharides, α-GOS), phytate, tannins, and alkaloids can impede the absorption of many critical nutrients and cause major physiological disorders. To enhance silage quality a...

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Autores principales: Pham, Hai-Ha-Thi, Kim, Do-Hyung, Nguyen, Thanh Luan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10171302/
https://www.ncbi.nlm.nih.gov/pubmed/37180381
http://dx.doi.org/10.3389/fpls.2023.1145041
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author Pham, Hai-Ha-Thi
Kim, Do-Hyung
Nguyen, Thanh Luan
author_facet Pham, Hai-Ha-Thi
Kim, Do-Hyung
Nguyen, Thanh Luan
author_sort Pham, Hai-Ha-Thi
collection PubMed
description Anti-nutritional factors (ANFs) substances in plant products, such as indigestible non-starchy polysaccharides (α-galactooligosaccharides, α-GOS), phytate, tannins, and alkaloids can impede the absorption of many critical nutrients and cause major physiological disorders. To enhance silage quality and its tolerance threshold for humans as well as other animals, ANFs must be reduced. This study aims to identify and compare the bacterial species/strains that are potential use for industrial fermentation and ANFs reduction. A pan-genome study of 351 bacterial genomes was performed, and binary data was processed to quantify the number of genes involved in the removal of ANFs. Among four pan-genomes analysis, all 37 tested Bacillus subtilis genomes had one phytate degradation gene, while 91 out of 150 Enterobacteriacae genomes harbor at least one genes (maximum three). Although, no gene encoding phytase detected in genomes of Lactobacillus and Pediococcus species, they have genes involving indirectly in metabolism of phytate-derivatives to produce Myo-inositol, an important compound in animal cells physiology. In contrast, genes related to production of lectin, tannase and saponin degrading enzyme did not include in genomes of B. subtilis and Pediococcus species. Our findings suggest a combination of bacterial species and/or unique strains in fermentation, for examples, two Lactobacillus strains (DSM 21115 and ATCC 14869) with B. subtilis SRCM103689, would maximize the efficiency in reducing the ANFs concentration. In conclusion, this study provides insights into bacterial genomes analysis for maximizing nutritional value in plant-based food. Further investigations of gene numbers and repertories correlated to metabolism of different ANFs will help clarifying the efficiency of time consuming and food qualities.
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spelling pubmed-101713022023-05-11 Wide-genome selection of lactic acid bacteria harboring genes that promote the elimination of antinutritional factors Pham, Hai-Ha-Thi Kim, Do-Hyung Nguyen, Thanh Luan Front Plant Sci Plant Science Anti-nutritional factors (ANFs) substances in plant products, such as indigestible non-starchy polysaccharides (α-galactooligosaccharides, α-GOS), phytate, tannins, and alkaloids can impede the absorption of many critical nutrients and cause major physiological disorders. To enhance silage quality and its tolerance threshold for humans as well as other animals, ANFs must be reduced. This study aims to identify and compare the bacterial species/strains that are potential use for industrial fermentation and ANFs reduction. A pan-genome study of 351 bacterial genomes was performed, and binary data was processed to quantify the number of genes involved in the removal of ANFs. Among four pan-genomes analysis, all 37 tested Bacillus subtilis genomes had one phytate degradation gene, while 91 out of 150 Enterobacteriacae genomes harbor at least one genes (maximum three). Although, no gene encoding phytase detected in genomes of Lactobacillus and Pediococcus species, they have genes involving indirectly in metabolism of phytate-derivatives to produce Myo-inositol, an important compound in animal cells physiology. In contrast, genes related to production of lectin, tannase and saponin degrading enzyme did not include in genomes of B. subtilis and Pediococcus species. Our findings suggest a combination of bacterial species and/or unique strains in fermentation, for examples, two Lactobacillus strains (DSM 21115 and ATCC 14869) with B. subtilis SRCM103689, would maximize the efficiency in reducing the ANFs concentration. In conclusion, this study provides insights into bacterial genomes analysis for maximizing nutritional value in plant-based food. Further investigations of gene numbers and repertories correlated to metabolism of different ANFs will help clarifying the efficiency of time consuming and food qualities. Frontiers Media S.A. 2023-04-26 /pmc/articles/PMC10171302/ /pubmed/37180381 http://dx.doi.org/10.3389/fpls.2023.1145041 Text en Copyright © 2023 Pham, Kim and Nguyen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Pham, Hai-Ha-Thi
Kim, Do-Hyung
Nguyen, Thanh Luan
Wide-genome selection of lactic acid bacteria harboring genes that promote the elimination of antinutritional factors
title Wide-genome selection of lactic acid bacteria harboring genes that promote the elimination of antinutritional factors
title_full Wide-genome selection of lactic acid bacteria harboring genes that promote the elimination of antinutritional factors
title_fullStr Wide-genome selection of lactic acid bacteria harboring genes that promote the elimination of antinutritional factors
title_full_unstemmed Wide-genome selection of lactic acid bacteria harboring genes that promote the elimination of antinutritional factors
title_short Wide-genome selection of lactic acid bacteria harboring genes that promote the elimination of antinutritional factors
title_sort wide-genome selection of lactic acid bacteria harboring genes that promote the elimination of antinutritional factors
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10171302/
https://www.ncbi.nlm.nih.gov/pubmed/37180381
http://dx.doi.org/10.3389/fpls.2023.1145041
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