Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+

Discovery of off-target CRISPR–Cas activity in patient-derived cells and animal models is crucial for genome editing applications, but currently exhibits low sensitivity. We demonstrate that inhibition of DNA-dependent protein kinase catalytic subunit accumulates the repair protein MRE11 at CRISPR–C...

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Detalles Bibliográficos
Autores principales: Zou, Roger S., Liu, Yang, Gaido, Oscar E. Reyes, Konig, Maximilian F., Mog, Brian J., Shen, Leo L., Aviles-Vazquez, Franklin, Marin-Gonzalez, Alberto, Ha, Taekjip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10172116/
https://www.ncbi.nlm.nih.gov/pubmed/37024653
http://dx.doi.org/10.1038/s41592-023-01840-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10172116/
https://www.ncbi.nlm.nih.gov/pubmed/37024653
http://dx.doi.org/10.1038/s41592-023-01840-z

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