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Isolation of human cutaneous immune cells for single-cell RNA sequencing

Single-cell RNA sequencing (scRNA-seq) allows for high-resolution analysis of transcriptionally dysregulated cell subpopulations in inflammatory diseases. However, it can be challenging to properly isolate viable immune cells from human skin for scRNA-seq due to its barrier properties. Here, we pres...

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Detalles Bibliográficos
Autores principales: Hailer, Ashley A., Wu, David, El Kurdi, Abdullah, Yuan, Michelle, Cho, Raymond J., Cheng, Jeffrey B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10173011/
https://www.ncbi.nlm.nih.gov/pubmed/37120815
http://dx.doi.org/10.1016/j.xpro.2023.102239