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Isolation of human cutaneous immune cells for single-cell RNA sequencing

Single-cell RNA sequencing (scRNA-seq) allows for high-resolution analysis of transcriptionally dysregulated cell subpopulations in inflammatory diseases. However, it can be challenging to properly isolate viable immune cells from human skin for scRNA-seq due to its barrier properties. Here, we pres...

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Detalles Bibliográficos
Autores principales:	Hailer, Ashley A., Wu, David, El Kurdi, Abdullah, Yuan, Michelle, Cho, Raymond J., Cheng, Jeffrey B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10173011/ https://www.ncbi.nlm.nih.gov/pubmed/37120815 http://dx.doi.org/10.1016/j.xpro.2023.102239

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