Lateral flow immunoassay for rapid and sensitive detection of dsRNA contaminants in in vitro-transcribed mRNA products

High purity is essential in mRNA-based therapeutic applications. A major contaminant of in vitro-transcribed (IVT) mRNA manufacturing is double-stranded RNA (dsRNA), which can induce severe anti-viral immune responses. Detection methods, such as agarose gel electrophoresis, ELISA, and dot-blot assay...

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Detalles Bibliográficos
Autores principales: Luo, Dengwang, Wu, Zhanfeng, Wang, Daming, Zhang, Jieli, Shao, Fei, Wang, Shuo, Cestellos-Blanco, Stefano, Xu, Dawei, Cao, Yuhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2023
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10173069/
https://www.ncbi.nlm.nih.gov/pubmed/37181450
http://dx.doi.org/10.1016/j.omtn.2023.04.005

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10173069/
https://www.ncbi.nlm.nih.gov/pubmed/37181450
http://dx.doi.org/10.1016/j.omtn.2023.04.005

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