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In vivo two-photon calcium imaging of cortical neurons in neonatal mice

In vivo calcium imaging is essential to elucidate unique synchronous activities observed in the developing brain. Here, we present a protocol to image and analyze activity patterns in neonatal mouse neocortex in a single-cell level. We describe steps for in utero electroporation, cranial window surg...

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Detalles Bibliográficos
Autores principales: Egashira, Takamitsu, Nakagawa-Tamagawa, Nao, Abzhanova, Elvira, Kawae, Yuzuki, Kohara, Ayami, Koitabashi, Ryoko, Mizuno, Hiromi, Mizuno, Hidenobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10173855/
https://www.ncbi.nlm.nih.gov/pubmed/37119143
http://dx.doi.org/10.1016/j.xpro.2023.102245