In vivo two-photon calcium imaging of cortical neurons in neonatal mice

In vivo calcium imaging is essential to elucidate unique synchronous activities observed in the developing brain. Here, we present a protocol to image and analyze activity patterns in neonatal mouse neocortex in a single-cell level. We describe steps for in utero electroporation, cranial window surg...

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Detalles Bibliográficos
Autores principales: Egashira, Takamitsu, Nakagawa-Tamagawa, Nao, Abzhanova, Elvira, Kawae, Yuzuki, Kohara, Ayami, Koitabashi, Ryoko, Mizuno, Hiromi, Mizuno, Hidenobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10173855/
https://www.ncbi.nlm.nih.gov/pubmed/37119143
http://dx.doi.org/10.1016/j.xpro.2023.102245
Descripción
Sumario:In vivo calcium imaging is essential to elucidate unique synchronous activities observed in the developing brain. Here, we present a protocol to image and analyze activity patterns in neonatal mouse neocortex in a single-cell level. We describe steps for in utero electroporation, cranial window surgery, two-photon imaging, and activity correlation analysis. This protocol facilitates the understanding of neuronal activities and activity-dependent circuit formation during development. For complete details on the use and execution of this protocol, please refer to Mizuno et al. (2014),(1) Mizuno et al. (2018a),(2) and Mizuno et al. (2018b).(3)

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