In vivo two-photon calcium imaging of cortical neurons in neonatal mice

In vivo calcium imaging is essential to elucidate unique synchronous activities observed in the developing brain. Here, we present a protocol to image and analyze activity patterns in neonatal mouse neocortex in a single-cell level. We describe steps for in utero electroporation, cranial window surg...

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Autores principales: Egashira, Takamitsu, Nakagawa-Tamagawa, Nao, Abzhanova, Elvira, Kawae, Yuzuki, Kohara, Ayami, Koitabashi, Ryoko, Mizuno, Hiromi, Mizuno, Hidenobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10173855/
https://www.ncbi.nlm.nih.gov/pubmed/37119143
http://dx.doi.org/10.1016/j.xpro.2023.102245
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author Egashira, Takamitsu
Nakagawa-Tamagawa, Nao
Abzhanova, Elvira
Kawae, Yuzuki
Kohara, Ayami
Koitabashi, Ryoko
Mizuno, Hiromi
Mizuno, Hidenobu
author_facet Egashira, Takamitsu
Nakagawa-Tamagawa, Nao
Abzhanova, Elvira
Kawae, Yuzuki
Kohara, Ayami
Koitabashi, Ryoko
Mizuno, Hiromi
Mizuno, Hidenobu
author_sort Egashira, Takamitsu
collection PubMed
description In vivo calcium imaging is essential to elucidate unique synchronous activities observed in the developing brain. Here, we present a protocol to image and analyze activity patterns in neonatal mouse neocortex in a single-cell level. We describe steps for in utero electroporation, cranial window surgery, two-photon imaging, and activity correlation analysis. This protocol facilitates the understanding of neuronal activities and activity-dependent circuit formation during development. For complete details on the use and execution of this protocol, please refer to Mizuno et al. (2014),(1) Mizuno et al. (2018a),(2) and Mizuno et al. (2018b).(3)
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spelling pubmed-101738552023-05-12 In vivo two-photon calcium imaging of cortical neurons in neonatal mice Egashira, Takamitsu Nakagawa-Tamagawa, Nao Abzhanova, Elvira Kawae, Yuzuki Kohara, Ayami Koitabashi, Ryoko Mizuno, Hiromi Mizuno, Hidenobu STAR Protoc Protocol In vivo calcium imaging is essential to elucidate unique synchronous activities observed in the developing brain. Here, we present a protocol to image and analyze activity patterns in neonatal mouse neocortex in a single-cell level. We describe steps for in utero electroporation, cranial window surgery, two-photon imaging, and activity correlation analysis. This protocol facilitates the understanding of neuronal activities and activity-dependent circuit formation during development. For complete details on the use and execution of this protocol, please refer to Mizuno et al. (2014),(1) Mizuno et al. (2018a),(2) and Mizuno et al. (2018b).(3) Elsevier 2023-04-27 /pmc/articles/PMC10173855/ /pubmed/37119143 http://dx.doi.org/10.1016/j.xpro.2023.102245 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Egashira, Takamitsu
Nakagawa-Tamagawa, Nao
Abzhanova, Elvira
Kawae, Yuzuki
Kohara, Ayami
Koitabashi, Ryoko
Mizuno, Hiromi
Mizuno, Hidenobu
In vivo two-photon calcium imaging of cortical neurons in neonatal mice
title In vivo two-photon calcium imaging of cortical neurons in neonatal mice
title_full In vivo two-photon calcium imaging of cortical neurons in neonatal mice
title_fullStr In vivo two-photon calcium imaging of cortical neurons in neonatal mice
title_full_unstemmed In vivo two-photon calcium imaging of cortical neurons in neonatal mice
title_short In vivo two-photon calcium imaging of cortical neurons in neonatal mice
title_sort in vivo two-photon calcium imaging of cortical neurons in neonatal mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10173855/
https://www.ncbi.nlm.nih.gov/pubmed/37119143
http://dx.doi.org/10.1016/j.xpro.2023.102245
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