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Expression and secretion of glycosylated barley oxalate oxidase in Pichia pastoris

Oxalate oxidase is an enzyme that degrades oxalate and is used in commercial urinary assays to measure oxalate levels. The objective of this study was to establish an enhanced expression system for secretion and purification of oxalate oxidase using Pichia pastoris. A codon optimized synthetic oxala...

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Detalles Bibliográficos
Autores principales: Donelan, William, Li, ShiWu, Dominguez-Gutierrez, Paul R., Anderson IV, Augustus, Yang, Li-Jun, Nguyen, Cuong, Canales, Benjamin K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10174515/
https://www.ncbi.nlm.nih.gov/pubmed/37167324
http://dx.doi.org/10.1371/journal.pone.0285556