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Expression and secretion of glycosylated barley oxalate oxidase in Pichia pastoris
Oxalate oxidase is an enzyme that degrades oxalate and is used in commercial urinary assays to measure oxalate levels. The objective of this study was to establish an enhanced expression system for secretion and purification of oxalate oxidase using Pichia pastoris. A codon optimized synthetic oxala...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10174515/ https://www.ncbi.nlm.nih.gov/pubmed/37167324 http://dx.doi.org/10.1371/journal.pone.0285556 |