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Small extracellular vesicles derived from four dimensional-culture of mesenchymal stem cells induce alternatively activated macrophages by upregulating IGFBP2/EGFR to attenuate inflammation in the spinal cord injury of rats

Effectively reducing the inflammatory response after spinal cord injury (SCI) is a challenging clinical problem and the subject of active investigation. This study employed a porous scaffold-based three dimensional long-term culture technique to obtain human umbilical cord mesenchymal stem cell (hUC...

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Autores principales: Wang, Junhua, Wei, Qingshuai, Yang, Yue, Che, Mingtian, Ma, Yuanhuan, Peng, Lizhi, Yu, Haiyang, Shi, Huijuan, He, Guanheng, Wu, Rongjie, Zeng, Ting, Zeng, Xiang, Ma, Wenbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176095/
https://www.ncbi.nlm.nih.gov/pubmed/37187882
http://dx.doi.org/10.3389/fbioe.2023.1146981
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author Wang, Junhua
Wei, Qingshuai
Yang, Yue
Che, Mingtian
Ma, Yuanhuan
Peng, Lizhi
Yu, Haiyang
Shi, Huijuan
He, Guanheng
Wu, Rongjie
Zeng, Ting
Zeng, Xiang
Ma, Wenbin
author_facet Wang, Junhua
Wei, Qingshuai
Yang, Yue
Che, Mingtian
Ma, Yuanhuan
Peng, Lizhi
Yu, Haiyang
Shi, Huijuan
He, Guanheng
Wu, Rongjie
Zeng, Ting
Zeng, Xiang
Ma, Wenbin
author_sort Wang, Junhua
collection PubMed
description Effectively reducing the inflammatory response after spinal cord injury (SCI) is a challenging clinical problem and the subject of active investigation. This study employed a porous scaffold-based three dimensional long-term culture technique to obtain human umbilical cord mesenchymal stem cell (hUC-MSC)-derived Small Extracellular Vesicles (sEVs) (three dimensional culture over time, the “4D-sEVs”). Moreover, the vesicle size, number, and inner protein concentrations of the MSC 4D-sEVs contained altered protein profiles compared with those derived from 2D culture conditions. A proteomics analysis suggested broad changes, especially significant upregulation of Epidermal Growth Factors Receptor (EGFR) and Insulin-like Growth Factor Binding Protein 2 (IGFBP2) in 4D-sEVs compared with 2D-sEVs. The endocytosis of 4D-sEVs allowed for the binding of EGFR and IGFBP2, leading to downstream STAT3 phosphorylation and IL-10 secretion and effective induction of macrophages/microglia polarization from the pro-inflammatory M1 to anti-inflammatory M2 phenotype, both in vitro and in the injured areas of rats with compressive/contusive SCI. The reduction in neuroinflammation after 4D-sEVs delivery to the injury site epicenter led to significant neuroprotection, as evidenced by the number of surviving spinal neurons. Therefore, applying this novel 4D culture-derived Small Extracellular Vesicles could effectively curb the inflammatory response and increase tissue repair after SCI.
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spelling pubmed-101760952023-05-13 Small extracellular vesicles derived from four dimensional-culture of mesenchymal stem cells induce alternatively activated macrophages by upregulating IGFBP2/EGFR to attenuate inflammation in the spinal cord injury of rats Wang, Junhua Wei, Qingshuai Yang, Yue Che, Mingtian Ma, Yuanhuan Peng, Lizhi Yu, Haiyang Shi, Huijuan He, Guanheng Wu, Rongjie Zeng, Ting Zeng, Xiang Ma, Wenbin Front Bioeng Biotechnol Bioengineering and Biotechnology Effectively reducing the inflammatory response after spinal cord injury (SCI) is a challenging clinical problem and the subject of active investigation. This study employed a porous scaffold-based three dimensional long-term culture technique to obtain human umbilical cord mesenchymal stem cell (hUC-MSC)-derived Small Extracellular Vesicles (sEVs) (three dimensional culture over time, the “4D-sEVs”). Moreover, the vesicle size, number, and inner protein concentrations of the MSC 4D-sEVs contained altered protein profiles compared with those derived from 2D culture conditions. A proteomics analysis suggested broad changes, especially significant upregulation of Epidermal Growth Factors Receptor (EGFR) and Insulin-like Growth Factor Binding Protein 2 (IGFBP2) in 4D-sEVs compared with 2D-sEVs. The endocytosis of 4D-sEVs allowed for the binding of EGFR and IGFBP2, leading to downstream STAT3 phosphorylation and IL-10 secretion and effective induction of macrophages/microglia polarization from the pro-inflammatory M1 to anti-inflammatory M2 phenotype, both in vitro and in the injured areas of rats with compressive/contusive SCI. The reduction in neuroinflammation after 4D-sEVs delivery to the injury site epicenter led to significant neuroprotection, as evidenced by the number of surviving spinal neurons. Therefore, applying this novel 4D culture-derived Small Extracellular Vesicles could effectively curb the inflammatory response and increase tissue repair after SCI. Frontiers Media S.A. 2023-04-28 /pmc/articles/PMC10176095/ /pubmed/37187882 http://dx.doi.org/10.3389/fbioe.2023.1146981 Text en Copyright © 2023 Wang, Wei, Yang, Che, Ma, Peng, Yu, Shi, He, Wu, Zeng, Zeng and Ma. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Wang, Junhua
Wei, Qingshuai
Yang, Yue
Che, Mingtian
Ma, Yuanhuan
Peng, Lizhi
Yu, Haiyang
Shi, Huijuan
He, Guanheng
Wu, Rongjie
Zeng, Ting
Zeng, Xiang
Ma, Wenbin
Small extracellular vesicles derived from four dimensional-culture of mesenchymal stem cells induce alternatively activated macrophages by upregulating IGFBP2/EGFR to attenuate inflammation in the spinal cord injury of rats
title Small extracellular vesicles derived from four dimensional-culture of mesenchymal stem cells induce alternatively activated macrophages by upregulating IGFBP2/EGFR to attenuate inflammation in the spinal cord injury of rats
title_full Small extracellular vesicles derived from four dimensional-culture of mesenchymal stem cells induce alternatively activated macrophages by upregulating IGFBP2/EGFR to attenuate inflammation in the spinal cord injury of rats
title_fullStr Small extracellular vesicles derived from four dimensional-culture of mesenchymal stem cells induce alternatively activated macrophages by upregulating IGFBP2/EGFR to attenuate inflammation in the spinal cord injury of rats
title_full_unstemmed Small extracellular vesicles derived from four dimensional-culture of mesenchymal stem cells induce alternatively activated macrophages by upregulating IGFBP2/EGFR to attenuate inflammation in the spinal cord injury of rats
title_short Small extracellular vesicles derived from four dimensional-culture of mesenchymal stem cells induce alternatively activated macrophages by upregulating IGFBP2/EGFR to attenuate inflammation in the spinal cord injury of rats
title_sort small extracellular vesicles derived from four dimensional-culture of mesenchymal stem cells induce alternatively activated macrophages by upregulating igfbp2/egfr to attenuate inflammation in the spinal cord injury of rats
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176095/
https://www.ncbi.nlm.nih.gov/pubmed/37187882
http://dx.doi.org/10.3389/fbioe.2023.1146981
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