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Computational Insights and In Silico Characterization of a Novel Mini-Lipoxygenase from Nostoc Sphaeroides and Its Application in the Quality Improvement of Steamed Bread
Lipoxygenase (EC1.13.11.12, LOX) has been potentially used in the food industry for food quality improvement. However, the low activity, poor thermal stability, narrow range of pH stability, as well as undesirable isoenzymes and off-flavors, have hampered the application of current commercial LOX. I...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177866/ https://www.ncbi.nlm.nih.gov/pubmed/37175648 http://dx.doi.org/10.3390/ijms24097941 |
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author | Xia, Bingjie Chi, Huibing Zhang, Bingjie Lu, Zhaoxin Liu, Huawei Lu, Fengxia Zhu, Ping |
author_facet | Xia, Bingjie Chi, Huibing Zhang, Bingjie Lu, Zhaoxin Liu, Huawei Lu, Fengxia Zhu, Ping |
author_sort | Xia, Bingjie |
collection | PubMed |
description | Lipoxygenase (EC1.13.11.12, LOX) has been potentially used in the food industry for food quality improvement. However, the low activity, poor thermal stability, narrow range of pH stability, as well as undesirable isoenzymes and off-flavors, have hampered the application of current commercial LOX. In this study, a putative mini-lipoxygenase gene from cyanobacteria, Nostoc sphaeroides (NsLOX), was cloned and expressed in E. coli BL21. NsLOX displayed only 26.62% structural identity with the reported LOX from Cyanothece sp., indicating it as a novel LOX. The purified NsLOX showed the maximum activity at pH 8.0 and 15 °C, with superior stability at a pH range from 6.0 to 13.0, retaining about 40% activity at 40 °C for 90 min. Notably, NsLOX exhibited the highest specific activity of 78,080 U/mg towards linoleic acid (LA), and the kinetic parameters—K(m), k(cat), and k(cat)/K(m)—attain values of 19.46 μM, 9199.75 s(−1), and 473.85 μM(−1) s(−1), respectively. Moreover, the activity of NsLOX was obviously activated by Ca(2+), but it was completely inhibited by Zn(2+) and Cu(2+). Finally, NsLOX was supplied in steamed bread and contributed even better improved bread quality than the commercial LOX. These results suggest NsLOX as a promising substitute of current commercial LOX for application in the food industry. |
format | Online Article Text |
id | pubmed-10177866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101778662023-05-13 Computational Insights and In Silico Characterization of a Novel Mini-Lipoxygenase from Nostoc Sphaeroides and Its Application in the Quality Improvement of Steamed Bread Xia, Bingjie Chi, Huibing Zhang, Bingjie Lu, Zhaoxin Liu, Huawei Lu, Fengxia Zhu, Ping Int J Mol Sci Article Lipoxygenase (EC1.13.11.12, LOX) has been potentially used in the food industry for food quality improvement. However, the low activity, poor thermal stability, narrow range of pH stability, as well as undesirable isoenzymes and off-flavors, have hampered the application of current commercial LOX. In this study, a putative mini-lipoxygenase gene from cyanobacteria, Nostoc sphaeroides (NsLOX), was cloned and expressed in E. coli BL21. NsLOX displayed only 26.62% structural identity with the reported LOX from Cyanothece sp., indicating it as a novel LOX. The purified NsLOX showed the maximum activity at pH 8.0 and 15 °C, with superior stability at a pH range from 6.0 to 13.0, retaining about 40% activity at 40 °C for 90 min. Notably, NsLOX exhibited the highest specific activity of 78,080 U/mg towards linoleic acid (LA), and the kinetic parameters—K(m), k(cat), and k(cat)/K(m)—attain values of 19.46 μM, 9199.75 s(−1), and 473.85 μM(−1) s(−1), respectively. Moreover, the activity of NsLOX was obviously activated by Ca(2+), but it was completely inhibited by Zn(2+) and Cu(2+). Finally, NsLOX was supplied in steamed bread and contributed even better improved bread quality than the commercial LOX. These results suggest NsLOX as a promising substitute of current commercial LOX for application in the food industry. MDPI 2023-04-27 /pmc/articles/PMC10177866/ /pubmed/37175648 http://dx.doi.org/10.3390/ijms24097941 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xia, Bingjie Chi, Huibing Zhang, Bingjie Lu, Zhaoxin Liu, Huawei Lu, Fengxia Zhu, Ping Computational Insights and In Silico Characterization of a Novel Mini-Lipoxygenase from Nostoc Sphaeroides and Its Application in the Quality Improvement of Steamed Bread |
title | Computational Insights and In Silico Characterization of a Novel Mini-Lipoxygenase from Nostoc Sphaeroides and Its Application in the Quality Improvement of Steamed Bread |
title_full | Computational Insights and In Silico Characterization of a Novel Mini-Lipoxygenase from Nostoc Sphaeroides and Its Application in the Quality Improvement of Steamed Bread |
title_fullStr | Computational Insights and In Silico Characterization of a Novel Mini-Lipoxygenase from Nostoc Sphaeroides and Its Application in the Quality Improvement of Steamed Bread |
title_full_unstemmed | Computational Insights and In Silico Characterization of a Novel Mini-Lipoxygenase from Nostoc Sphaeroides and Its Application in the Quality Improvement of Steamed Bread |
title_short | Computational Insights and In Silico Characterization of a Novel Mini-Lipoxygenase from Nostoc Sphaeroides and Its Application in the Quality Improvement of Steamed Bread |
title_sort | computational insights and in silico characterization of a novel mini-lipoxygenase from nostoc sphaeroides and its application in the quality improvement of steamed bread |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177866/ https://www.ncbi.nlm.nih.gov/pubmed/37175648 http://dx.doi.org/10.3390/ijms24097941 |
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