Protocol to isolate temperature-sensitive SARS-CoV-2 mutants and identify associated mutations

An inability to proliferate at high temperatures typically gives viruses an attenuated phenotype. Here, we present a protocol to obtain and isolate temperature-sensitive (TS) SARS-CoV-2 strains via 5-fluorouracile-induced mutagenesis. We describe steps for the induction of mutations in the wild-type...

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Detalles Bibliográficos
Autores principales: Okamura, Shinya, Yoshida, Akiho, Miyazato, Paola, Matsumoto, Mai, Ebina, Hirotaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183609/
https://www.ncbi.nlm.nih.gov/pubmed/37300825
http://dx.doi.org/10.1016/j.xpro.2023.102352

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183609/
https://www.ncbi.nlm.nih.gov/pubmed/37300825
http://dx.doi.org/10.1016/j.xpro.2023.102352

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