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Protocol to isolate temperature-sensitive SARS-CoV-2 mutants and identify associated mutations
An inability to proliferate at high temperatures typically gives viruses an attenuated phenotype. Here, we present a protocol to obtain and isolate temperature-sensitive (TS) SARS-CoV-2 strains via 5-fluorouracile-induced mutagenesis. We describe steps for the induction of mutations in the wild-type...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183609/ https://www.ncbi.nlm.nih.gov/pubmed/37300825 http://dx.doi.org/10.1016/j.xpro.2023.102352 |