Cargando…

Influence of trans‐ferulic acid on the quality of ram semen upon cold preservation

BACKGROUND: Due to lower antioxidant capacity and higher amounts of polyunsaturated fatty acids, ram spermatozoa are very susceptible during cooling process. OBJECTIVES: The objective was to examine the effect of the trans‐ferulic acid (t‐FA) on the ram semen during liquid preservation. METHODS: Sem...

Descripción completa

Detalles Bibliográficos
Autores principales: Salimi, Ali, Eslami, Mohsen, Farrokhi‐Ardabili, Farhad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10188079/
https://www.ncbi.nlm.nih.gov/pubmed/36913307
http://dx.doi.org/10.1002/vms3.1117
_version_ 1785042838026715136
author Salimi, Ali
Eslami, Mohsen
Farrokhi‐Ardabili, Farhad
author_facet Salimi, Ali
Eslami, Mohsen
Farrokhi‐Ardabili, Farhad
author_sort Salimi, Ali
collection PubMed
description BACKGROUND: Due to lower antioxidant capacity and higher amounts of polyunsaturated fatty acids, ram spermatozoa are very susceptible during cooling process. OBJECTIVES: The objective was to examine the effect of the trans‐ferulic acid (t‐FA) on the ram semen during liquid preservation. METHODS: Semen samples were collected from the Qezel rams, pooled, and extended with the Tris‐based diluent. Pooled samples enriched with different amounts of the t‐FA (0, 2.5, 5, 10, and 25 mM) and preserved at 4°C for 72 h. Spermatozoa's kinematics, membrane functionality, and viability were assessed by CASA system, hypoosmotic swelling test, and eosin‐nigrosin staining, respectively. Moreover, biochemical parameters were measured at 0, 24, 48, and 72 h. RESULTS: Results showed that 5 and 10 mM t‐FA improved forward progressive motility (FPM) and curvilinear velocity compared to the other groups at 72 h (p < 0.05). Samples treated with 25 mM t‐FA showed the lowest total motility, FPM, and viability at 24, 48, and 72 h of storage (p < 0.05). Higher total antioxidant activity levels were observed in the 10 mM t‐FA‐treated group compared to the negative control at 72 h (p < 0.05). Treatment with 25 mM t‐FA increased malondialdehyde amounts and decreased superoxide dismutase activity compared to other groups at the final time assessment (p < 0.05). Nitrate‐nitrite and lipid hydroperoxides values were not affected by treatment. CONCLUSIONS: The current study indicates the positive and negative influences of different concentrations of t‐FA on the ram semen upon cold storage.
format Online
Article
Text
id pubmed-10188079
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-101880792023-05-17 Influence of trans‐ferulic acid on the quality of ram semen upon cold preservation Salimi, Ali Eslami, Mohsen Farrokhi‐Ardabili, Farhad Vet Med Sci RUMINANTS BACKGROUND: Due to lower antioxidant capacity and higher amounts of polyunsaturated fatty acids, ram spermatozoa are very susceptible during cooling process. OBJECTIVES: The objective was to examine the effect of the trans‐ferulic acid (t‐FA) on the ram semen during liquid preservation. METHODS: Semen samples were collected from the Qezel rams, pooled, and extended with the Tris‐based diluent. Pooled samples enriched with different amounts of the t‐FA (0, 2.5, 5, 10, and 25 mM) and preserved at 4°C for 72 h. Spermatozoa's kinematics, membrane functionality, and viability were assessed by CASA system, hypoosmotic swelling test, and eosin‐nigrosin staining, respectively. Moreover, biochemical parameters were measured at 0, 24, 48, and 72 h. RESULTS: Results showed that 5 and 10 mM t‐FA improved forward progressive motility (FPM) and curvilinear velocity compared to the other groups at 72 h (p < 0.05). Samples treated with 25 mM t‐FA showed the lowest total motility, FPM, and viability at 24, 48, and 72 h of storage (p < 0.05). Higher total antioxidant activity levels were observed in the 10 mM t‐FA‐treated group compared to the negative control at 72 h (p < 0.05). Treatment with 25 mM t‐FA increased malondialdehyde amounts and decreased superoxide dismutase activity compared to other groups at the final time assessment (p < 0.05). Nitrate‐nitrite and lipid hydroperoxides values were not affected by treatment. CONCLUSIONS: The current study indicates the positive and negative influences of different concentrations of t‐FA on the ram semen upon cold storage. John Wiley and Sons Inc. 2023-03-13 /pmc/articles/PMC10188079/ /pubmed/36913307 http://dx.doi.org/10.1002/vms3.1117 Text en © 2023 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle RUMINANTS
Salimi, Ali
Eslami, Mohsen
Farrokhi‐Ardabili, Farhad
Influence of trans‐ferulic acid on the quality of ram semen upon cold preservation
title Influence of trans‐ferulic acid on the quality of ram semen upon cold preservation
title_full Influence of trans‐ferulic acid on the quality of ram semen upon cold preservation
title_fullStr Influence of trans‐ferulic acid on the quality of ram semen upon cold preservation
title_full_unstemmed Influence of trans‐ferulic acid on the quality of ram semen upon cold preservation
title_short Influence of trans‐ferulic acid on the quality of ram semen upon cold preservation
title_sort influence of trans‐ferulic acid on the quality of ram semen upon cold preservation
topic RUMINANTS
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10188079/
https://www.ncbi.nlm.nih.gov/pubmed/36913307
http://dx.doi.org/10.1002/vms3.1117
work_keys_str_mv AT salimiali influenceoftransferulicacidonthequalityoframsemenuponcoldpreservation
AT eslamimohsen influenceoftransferulicacidonthequalityoframsemenuponcoldpreservation
AT farrokhiardabilifarhad influenceoftransferulicacidonthequalityoframsemenuponcoldpreservation