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Protocol for ex vivo culture of patient-derived tumor fragments

The lack of suitable models currently hampers our understanding of how the tumor microenvironment responds to immunotherapy treatment. Here, we present a protocol for ex vivo culture of patient-derived tumor fragments (PDTFs). We describe the steps for tumor collection, generation and cryopreservati...

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Detalles Bibliográficos
Autores principales: Roelofsen, Lisanne M., Voabil, Paula, de Bruijn, Marjolein, Herzig, Petra, Zippelius, Alfred, Schumacher, Ton N., Thommen, Daniela S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189508/
https://www.ncbi.nlm.nih.gov/pubmed/37149855
http://dx.doi.org/10.1016/j.xpro.2023.102282