Protocol for ex vivo culture of patient-derived tumor fragments

The lack of suitable models currently hampers our understanding of how the tumor microenvironment responds to immunotherapy treatment. Here, we present a protocol for ex vivo culture of patient-derived tumor fragments (PDTFs). We describe the steps for tumor collection, generation and cryopreservati...

Descripción completa

Detalles Bibliográficos
Autores principales: Roelofsen, Lisanne M., Voabil, Paula, de Bruijn, Marjolein, Herzig, Petra, Zippelius, Alfred, Schumacher, Ton N., Thommen, Daniela S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189508/
https://www.ncbi.nlm.nih.gov/pubmed/37149855
http://dx.doi.org/10.1016/j.xpro.2023.102282
_version_ 1785043103678201856
author Roelofsen, Lisanne M.
Voabil, Paula
de Bruijn, Marjolein
Herzig, Petra
Zippelius, Alfred
Schumacher, Ton N.
Thommen, Daniela S.
author_facet Roelofsen, Lisanne M.
Voabil, Paula
de Bruijn, Marjolein
Herzig, Petra
Zippelius, Alfred
Schumacher, Ton N.
Thommen, Daniela S.
author_sort Roelofsen, Lisanne M.
collection PubMed
description The lack of suitable models currently hampers our understanding of how the tumor microenvironment responds to immunotherapy treatment. Here, we present a protocol for ex vivo culture of patient-derived tumor fragments (PDTFs). We describe the steps for tumor collection, generation and cryopreservation of PDTFs, and their subsequent thawing. We detail culture of PDTFs and their preparation for analysis. This protocol preserves the tumor microenvironment’s composition, architecture, and cellular interactions, which can be perturbed by ex vivo treatment. For complete details on the use and execution of this protocol, please refer to Voabil et al. (2021).(1)
format Online
Article
Text
id pubmed-10189508
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-101895082023-05-18 Protocol for ex vivo culture of patient-derived tumor fragments Roelofsen, Lisanne M. Voabil, Paula de Bruijn, Marjolein Herzig, Petra Zippelius, Alfred Schumacher, Ton N. Thommen, Daniela S. STAR Protoc Protocol The lack of suitable models currently hampers our understanding of how the tumor microenvironment responds to immunotherapy treatment. Here, we present a protocol for ex vivo culture of patient-derived tumor fragments (PDTFs). We describe the steps for tumor collection, generation and cryopreservation of PDTFs, and their subsequent thawing. We detail culture of PDTFs and their preparation for analysis. This protocol preserves the tumor microenvironment’s composition, architecture, and cellular interactions, which can be perturbed by ex vivo treatment. For complete details on the use and execution of this protocol, please refer to Voabil et al. (2021).(1) Elsevier 2023-05-06 /pmc/articles/PMC10189508/ /pubmed/37149855 http://dx.doi.org/10.1016/j.xpro.2023.102282 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Roelofsen, Lisanne M.
Voabil, Paula
de Bruijn, Marjolein
Herzig, Petra
Zippelius, Alfred
Schumacher, Ton N.
Thommen, Daniela S.
Protocol for ex vivo culture of patient-derived tumor fragments
title Protocol for ex vivo culture of patient-derived tumor fragments
title_full Protocol for ex vivo culture of patient-derived tumor fragments
title_fullStr Protocol for ex vivo culture of patient-derived tumor fragments
title_full_unstemmed Protocol for ex vivo culture of patient-derived tumor fragments
title_short Protocol for ex vivo culture of patient-derived tumor fragments
title_sort protocol for ex vivo culture of patient-derived tumor fragments
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189508/
https://www.ncbi.nlm.nih.gov/pubmed/37149855
http://dx.doi.org/10.1016/j.xpro.2023.102282
work_keys_str_mv AT roelofsenlisannem protocolforexvivocultureofpatientderivedtumorfragments
AT voabilpaula protocolforexvivocultureofpatientderivedtumorfragments
AT debruijnmarjolein protocolforexvivocultureofpatientderivedtumorfragments
AT herzigpetra protocolforexvivocultureofpatientderivedtumorfragments
AT zippeliusalfred protocolforexvivocultureofpatientderivedtumorfragments
AT schumachertonn protocolforexvivocultureofpatientderivedtumorfragments
AT thommendanielas protocolforexvivocultureofpatientderivedtumorfragments

Ejemplares similares