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Protocol for ex vivo culture of patient-derived tumor fragments
The lack of suitable models currently hampers our understanding of how the tumor microenvironment responds to immunotherapy treatment. Here, we present a protocol for ex vivo culture of patient-derived tumor fragments (PDTFs). We describe the steps for tumor collection, generation and cryopreservati...
Autores principales: | Roelofsen, Lisanne M., Voabil, Paula, de Bruijn, Marjolein, Herzig, Petra, Zippelius, Alfred, Schumacher, Ton N., Thommen, Daniela S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189508/ https://www.ncbi.nlm.nih.gov/pubmed/37149855 http://dx.doi.org/10.1016/j.xpro.2023.102282 |
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