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Direct Nanopore Sequencing of Individual Full Length tRNA Strands

[Image: see text] We describe a method for direct tRNA sequencing using the Oxford Nanopore MinION. The principal technical advance is custom adapters that facilitate end-to-end sequencing of individual transfer RNA (tRNA) molecules at subnanometer precision. A second advance is a nanopore sequencin...

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Detalles Bibliográficos
Autores principales: Thomas, Niki K., Poodari, Vinay C., Jain, Miten, Olsen, Hugh E., Akeson, Mark, Abu-Shumays, Robin L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189790/
https://www.ncbi.nlm.nih.gov/pubmed/34618430
http://dx.doi.org/10.1021/acsnano.1c06488